The Single Best Strategy To Use For flow cytometer
The Single Best Strategy To Use For flow cytometer
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Info-enriched characterization of cell populations. Morphological data from photos can incorporate towards the richness of flow cytometry details. One example is, the determine shows an in any other case common apoptosis assay using Annexin V and PI, introducing cell imaging to characterize cells in Every inhabitants to expose morphologically distinct characteristics. These insights couldn't are acquired from multiplex staining by itself.
As samples are acquired around the Attune CytPix Flow Cytometer, the superior-velocity brightfield digicam captures and shops images of detected gatherings in a amount as much as six,000 images for each next, with regards to the flow charge and impression size. For bigger flexibility, Attune Cytometric Software allows you to modify the picture seize frequency as desired and select a particular gate for picture seize, if essential.
Combine acoustic focusing engineering with higher-parameter Assessment for adaptable spectral unmixing and standard workflows.
Explore new insights into your experimental knowledge by visualizing populations utilizing combined fluorescence and morphology information. Our software program interface wizards help downsampling, concatenation (merged documents) and dimensionality instruments which can be designed in to the application for an intuitive, streamlined information Examination working experience.
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Furthermore, it is illuminating to find out the quantity of doublets slip via conventional singlet gates, and good to verify the status of unforeseen double-optimistic events in my Assessment."
Pair your Attune flow cytometer using an autosampler for improved workflow performance, and incorporate robotic integration for max throughput.
Dependable picture quality even at superior throughput Acoustic focusing and also a substantial-speed camera Blend to impression these T cells regularly at lower or high flow premiums. Effortlessly adjust target and digicam settings to fulfill experimental requirements.
Early signs of this contamination had to begin with been dismissed as particles. Backgating to find new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also lets you use morphological characteristics to find out interesting subpopulations that will not be evident from flow cytometry information by itself.
Potentially most of all, these situations consist of cells of Obviously various phenotypes which could lead on to incorrect conclusions pertaining to double positive activities (particularly in rare populations).
AI-pushed automatic impression Examination Using the Attune Cytometric Software translates function attributes into distinctive morphology parameters that could be coupled with standard fluorescence and scatter parameters to reveal very reproducible inhabitants adjustments and one of a kind subsets.
Software Observe: Automatic graphic Investigation lowers user-to-person variability in flow cytometry gating strategies
Designed-in dimensionality reduction resources increased resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to confirm predicted populace resolution in lysed full human blood. Person can check out activities from Every cluster for visual affirmation of mobile phenotypes.
Flexible payment plan from Agilent Monetary Solutions allow you to have the significant instrumentation you will need for speedy venture, or to expand your lab.
At the heart of each Attune Flow Cytometer lies our revolutionary acoustic concentrating technological innovation, which utilizes seem waves to meticulously align cells within the flow mobile. This progressive tactic surpasses traditional hydrodynamic approaches, resulting in speedier processing speeds, Extraordinary knowledge high-quality, and elevated sample throughput.
The Attune Xenith cytometer can be a significant-parameter spectral analyzer, providing both spectral unmixing and standard flow abilities for complete and multipurpose mobile Investigation.
The Attune CytPix Flow Cytometer allows users to visualize samples through acquisition, read more validate the precision of gating techniques, and guidance results with dependable documentation for publication purposes.
What seemed to be a homogeneous populace is actually extra assorted—an Perception that ought to be regarded when interpreting final results.